Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams

The current study aimed to examine the percentage changes of viability sperm and activity enzymes LDH, ALP, GOT / AST, GPT ALT in plasma Lacaune rams, before after cryopreservation. For this purpose, five rams were examined, two ejaculates obtained from each ram. Ejaculates are collected by method artificial vagina, during insemination campaign. All diluted with a 6AG extender frozen Cassou?s sequin method. Sperm was determined eosin nigrosine smears, enzyme examined spectrophotometrically. As result, vital cryopreservation decreased 15% (P ? 0.001). freezing thawing process also reduced AST ALP. In conclusion, observed enzymes, relation vitality, could be used as indicators optimize protocols for ram?s sperm.